A novel preparative method for the isolation of avidin and riboflavin-binding glycoprotein from chicken egg-white by the use of high-performance liquid chromatography.

نویسندگان

  • V E Piskarev
  • A M Shuster
  • A G Gabibov
  • A G Rabinkov
چکیده

A method for the rapid preparative isolation of highly purified avidin using h.p.l.c. on a powerful cation-exchanger TSK SP-5PW column has been developed. The method is based on the following properties of avidin: solubility at a high (NH4)2SO4 concentration, stability and relatively low solubility in organic solvents, as well as the strongly cationic nature of the molecule. Riboflavin-binding glycoprotein may be isolated as by-product.

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عنوان ژورنال:
  • The Biochemical journal

دوره 265 1  شماره 

صفحات  -

تاریخ انتشار 1990